Nikon film scan (Slides of microscopic specimens)

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Scanner hardware

Nikon Super Coolscan 5000 ED (film scanner)

Specifications

  • Scanning system: Fixed film, movable plane single-pass optical scanning system
  • Light source: R, G, B and Infrared (IR) LEDs
  • Image sensor: 3,964-pixel, two-line linear CCD image sensor
  • Color separation: Performed by RGB LEDs
  • Optical resolution: Up to 4,000 pixels per inch
  • A/D conversion: 16 bits per color
  • Density range: 4.8
  • Output: Full color or greyscale at 8 or 16 bits per channel
  • Focus: Auto and manual; autofocusing point selectable

Processable media

Negatives and positives, in color and monochrome

  • 35mm slides
    • Slides with mounts 1.0 - 3.2mm thick, 49 - 50.8mm wide; optional SLIDE FEEDER SF-210 can be used to scan up to 50 slides with mounts 1.5mm thick
  • 35mm film strips
    • 2 - 6 frames (2 - 40 frames with optional ROLL FILM ADAPTER SA-30); strips of 1 - 6 frames can be scanned with optional STRIP FILM HOLDER FH-3
  • APS (IX240) film
    • Slides with mounts 1.0 - 3.2mm thick can be scanned with optional 120/220 MOUNTED FILM HOLDER FH-869M
  • Preparates (slide glass for microscope)
    • Prepared slides (26 x 76mm, 0.8 - 1.5 mm thick) can be scanned with optional MEDICAL SLIDE HOLDER FH-G1

Additional equipment

  • Slide feeder SF-210
  • Strip film holder FH-3
  • Medical slide holder FH-G1

Digitizing server infrastructure

Digitization workstation - computer hardware and software

  • Intel Core i3-2310M 2.10 GHz
  • 3.1 GB RAM
  • 500 GB hard disk
  • 1428 MB Graphic Card
  • MS Windows 7
  • Adobe Photoshop CS4
  • MySQL database + phpMyAdmin
  • Silverfast AI + HDRStudio

Storage server and backup

  • CIFS share on DualHead NAS (on EMC Celerra NS120 80TB)
  • Backup via NDM protocol to LTO5 tape library (Dell ML6000)

Image server (publishing the tif files for the WWW)

Digitizing workflow

  • Specimen data inserted/extended in MySQL database
  • Dia slides are scanned via Nikon Coolscan 5000 and Silverfast
  • Image saved as an uncompressed tiff file with ~ 100 MB, internal accession numbers as filename
  • Adjusting contrast and brightness, further processing e.g. dust and scratch removal if required
  • Image saved as a compressed tiff file with ~ 50 MB for web publishing, internal accession numbers as filename

--Carola Söhngen 08:22, 20 June 2012 (CEST)