Hochauflösende Kameraeinrichtung mit softwaregesteuert bewegbarem Tisch optimiert für dreidimensionale Makroobjekte (Kleinpilze) und hohe Schärfentiefe

High-resolution camera system with software-controlled movable table optimized for three-dimensional macro objects (small mushrooms) and high depth of field

http://species-id.net/o/index.php?title=File:High_Resolution_Camera_System_Sample_Scanner_2013-09-26.jpg http://species-id.net/o/index.php?title=File:High_Resolution_Camera_System_Example_2013-09-26.jpg

Camera hardware

• DSLR: Nikon D2X (12.5 Megapixel)
• Macro lens: Micro Nikkor 60 mm 1:2.8 D

Lighting

• 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter)
• 2 x LED-5001 Video Light (5500 K, 700 lx, diffusor filter)

Digitization workstation - computer hardware and software

Computer hardware:

• Intel Core i7-870 CPU (8M Cache, 2.93 GHz)
• 8 GB RAM
• 2 x Western Digital WD5002ABYS-02B1B0 (500 GB, SATA2) RAID 0 HDD
• NVIDIA Quadro 2000 graphics card (1 GB DDR5 frame buffer)
• QUATO Intelli Proof 242 ex 24" proof monitor
• Handheld barcode scanner (USB)

Electric sample table (XYZ-Sample-Scanner):

• Software-controlled table (custom product by the Elektronikwerkstatt Universität Bayreut), automatically adjustable in three dimensions with micrometre precision by means of electrical stepping motors

Software:

• Windows 7 Ultimate 64 Bit
• Nikon Camera Control Pro 1.3.0
• Elub 0781 2.1 control software (custom product by P. Reiss, Elektronikwerkstatt Universität Bayreuth)
• PhotoAcute 3
• Adobe Photoshop 12.0.4

Digitizing server infrastructure

Storage server for images, image web publication server, web server, database server - at the SNSB IT Center

Digitizing workflow

• The object to be digitized is placed on the electric table
• Up to 24 single photographs are automatically taken by the Elub 0781 software thereby slightly changing the focus settings after each shot according to predefined parameters
• The single shots (RAW files) are further processed to one new image applying superresolution and focus stacking technologies provided by the Photo Acute software
• The resulting artificial image contains only that parts of the underlying single shots, which are in focus (focus stacking), and its resolution is enhanced approximately fourfold (superresolution)
• This final raw image file is colour-adjusted, cropped, and exported as TIFF in Photoshop
• Single shot raw files, combined raw file, and TIFFs are stored at the Leibniz Rechenzentrum München

Contact person(s)

Sebastian Werner