Difference between revisions of "Hochauflösende Kameraeinrichtung mit softwaregesteuert bewegbarem Tisch optimiert für dreidimensionale Makroobjekte (Kleinpilze) und hohe Schärfentiefe"
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=== Lighting === | === Lighting === | ||
+ | Until December 2014 | ||
+ | * 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter) | ||
+ | * 2 x LED-5001 Video Light (5500 K, 700 lx, diffusor filter) | ||
+ | Since January 2015 | ||
* 2 x F&V 5600K-3200K Bi-color Flood K4000s LED Studio Light Panel (diffusor filter) | * 2 x F&V 5600K-3200K Bi-color Flood K4000s LED Studio Light Panel (diffusor filter) | ||
* 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter) | * 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter) |
Revision as of 10:10, 19 March 2015
High-resolution camera system with software-controlled movable table optimized for three-dimensional macro objects (small fungi) and high depth of field
Camera hardware
- DSLR: Nikon D2X (12.5 Megapixel)
- Macro lens: Micro Nikkor 60 mm 1:2.8 D
Lighting
Until December 2014
- 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter)
- 2 x LED-5001 Video Light (5500 K, 700 lx, diffusor filter)
Since January 2015
- 2 x F&V 5600K-3200K Bi-color Flood K4000s LED Studio Light Panel (diffusor filter)
- 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter)
Digitization workstation - hardware and software
Computer hardware:
- Intel Core i7-870 CPU (8M Cache, 2.93 GHz)
- 8 GB RAM
- 2 x Western Digital WD5002ABYS-02B1B0 (500 GB, SATA2) RAID 0 HDD
- NVIDIA Quadro 2000 graphics card (1 GB DDR5 frame buffer)
- QUATO Intelli Proof 242 ex 24" proof monitor
- Handheld barcode scanner (USB)
Electric sample table (XYZ-Sample-Scanner):
- Software-controlled table (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreut), automatically adjustable in three dimensions with micrometre precision by means of electrical stepping motors
Software:
- Windows 7 Ultimate 64 Bit
- Nikon Camera Control Pro 1.3.0
- Elub 0781 2.1 control software (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreuth)
- PhotoAcute 3
- Adobe Photoshop 12.0.4
Digitizing server infrastructure
Storage server for images, image web publication server, web server, database server - at the SNSB IT Center
Digitizing workflow
- The object to be digitized is placed on the electric table.
- Up to 30 single photographs are automatically taken by the Elub 0781 software thereby slightly changing the focus settings after each shot according to predefined parameters. This procedure takes about 10 minutes.
- The single shots (RAW files) are further processed to one new image applying superresolution and focus stacking technologies provided by PhotoAcute, which takes about 20 minutes.
- The resulting artificial image contains only that parts of the underlying single shots, which are in focus (focus stacking), and its resolution is enhanced approximately fourfold (superresolution).
- In Photoshop, this final raw image file is color-adjusted, cropped, and exported as TIFF, which takes about 5 minutes.
- Single shot raw files, combined raw file, and TIFFs are stored at the Leibniz Rechenzentrum München.
Contact person(s)
Sebastian Werner
Dr. Tobias Lehmann
Dr. Konstanze Bensch
Publication
- Werner, S., Bensch, K., Beck, A., Neubacher, D., Reichert, W., Reiß, P. & Triebel, D. 2014. Multi-layered digitization of research-relevant objects using focus stacking technologies and a software-controlled movable table. – In: Mallison, H., Vogel, J. & Belvedere, M. (eds.): Digital Specimen 2014 – Abstracts of Presentations. Museum für Naturkunde Berlin. – Abstract and Poster.