Difference between revisions of "Hochauflösende Kameraeinrichtung mit softwaregesteuert bewegbarem Tisch optimiert für dreidimensionale Makroobjekte (Kleinpilze) und hohe Schärfentiefe"

Revision as of 15:12, 16 March 2015

2 x F&V 5600K-3200K Bi-color Flood K4000s LED Studio Light Panel (diffusor filter)
1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter)

Computer hardware:

Electric sample table (XYZ-Sample-Scanner):

Software-controlled table (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreut), automatically adjustable in three dimensions with micrometre precision by means of electrical stepping motors

Software:

Windows 7 Ultimate 64 Bit
Nikon Camera Control Pro 1.3.0
Elub 0781 2.1 control software (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreuth)
PhotoAcute 3
Adobe Photoshop 12.0.4

Storage server for images, image web publication server, web server, database server - at the SNSB IT Center

The object to be digitized is placed on the electric table.
Up to 30 single photographs are automatically taken by the Elub 0781 software thereby slightly changing the focus settings after each shot according to predefined parameters. This procedure takes about 10 minutes.
The single shots (RAW files) are further processed to one new image applying superresolution and focus stacking technologies provided by PhotoAcute, which takes about 20 minutes.
The resulting artificial image contains only that parts of the underlying single shots, which are in focus (focus stacking), and its resolution is enhanced approximately fourfold (superresolution).
In Photoshop, this final raw image file is color-adjusted, cropped, and exported as TIFF, which takes about 5 minutes.
Single shot raw files, combined raw file, and TIFFs are stored at the Leibniz Rechenzentrum München.

Sebastian Werner

Dr. Tobias Lehmann

Dr. Konstanze Bensch

Werner, S., Bensch, K., Beck, A., Neubacher, D., Reichert, W., Reiß, P. & Triebel, D. 2014. Multi-layered digitization of research-relevant objects using focus stacking technologies and a software-controlled movable table. – In: Mallison, H., Vogel, J. & Belvedere, M. (eds.): Digital Specimen 2014 – Abstracts of Presentations. Museum für Naturkunde Berlin. – Abstract and Poster.

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 === Digitizing workflow ===
 * The object to be digitized is placed on the electric table.
-* Up to 24 single photographs are automatically taken by the Elub 0781 software thereby slightly changing the focus settings after each shot according to predefined parameters. This procedure takes about 10 minutes.
+* Up to 30 single photographs are automatically taken by the Elub 0781 software thereby slightly changing the focus settings after each shot according to predefined parameters. This procedure takes about 10 minutes.
 * The single shots (RAW files) are further processed to one new image applying superresolution and focus stacking technologies provided by PhotoAcute, which takes about 20 minutes.
 * The resulting artificial image contains only that parts of the underlying single shots, which are in focus (focus stacking), and its resolution is enhanced approximately fourfold (superresolution).