View source for Hochauflösende Kameraeinrichtung mit softwaregesteuert bewegbarem Tisch optimiert für dreidimensionale Makroobjekte (Kleinpilze) und hohe Schärfentiefe

== High-resolution camera system with software-controlled movable table optimized for three-dimensional macro objects (small fungi) and high depth of field ==
[[File:High Resolution Camera System Sample Scanner 2014-04-10.jpg|814px]]
[[File:High_Resolution_Camera_System_Example_2013-09-26.jpg|269px]]
[[File:High_Resolution_Camera_System_Example2_2013-09-27.jpg|545px]]

=== Camera hardware ===
* DSLR: Nikon D2X (12.5 Megapixel)
* Macro lens: Micro Nikkor 60 mm 1:2.8 D

=== Lighting ===
* 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter)
* 2 x LED-5001 Video Light (5500 K, 700 lx, diffusor filter)

=== Digitization workstation - hardware and software ===
Computer hardware:
* Intel Core i7-870 CPU (8M Cache, 2.93 GHz)
* 8 GB RAM
* 2 x Western Digital WD5002ABYS-02B1B0 (500 GB, SATA2) RAID 0 HDD
* NVIDIA Quadro 2000 graphics card (1 GB DDR5 frame buffer)
* QUATO Intelli Proof 242 ex 24" proof monitor
* Handheld barcode scanner (USB)

Electric sample table (XYZ-Sample-Scanner):
* Software-controlled table (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreut), automatically adjustable in three dimensions with micrometre precision by means of electrical stepping motors

Software:
* Windows 7 Ultimate 64 Bit
* Nikon Camera Control Pro 1.3.0
* Elub 0781 2.1 control software (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreuth)
* PhotoAcute 3
* Adobe Photoshop 12.0.4

=== Digitizing server infrastructure ===
Storage server for images, image web publication server, web server, database server 
- at the SNSB IT Center
=== Digitizing workflow ===
* The object to be digitized is placed on the electric table.
* Up to 24 single photographs are automatically taken by the Elub 0781 software thereby slightly changing the focus settings after each shot according to predefined parameters. This procedure takes about 10 minutes.
* The single shots (RAW files) are further processed to one new image applying superresolution and focus stacking technologies provided by PhotoAcute, which takes about 20 minutes.
* The resulting artificial image contains only that parts of the underlying single shots, which are in focus (focus stacking), and its resolution is enhanced approximately fourfold (superresolution).
* In Photoshop, this final raw image file is color-adjusted, cropped, and exported as TIFF, which takes about 5 minutes.
* Single shot raw files, combined raw file, and TIFFs are stored  at the Leibniz Rechenzentrum München.

=== Contact person(s) ===
Sebastian Werner

Dr. Konstanze Bensch

=== Publication ===

*Werner, S., Bensch, K., Beck, A., Neubacher, D., Reichert, W., Reiß, P. & Triebel, D. 2014. Multi-layered digitization of research-relevant objects using focus stacking technologies and a software-controlled movable table. – In: Mallison, H., Vogel, J. & Belvedere, M. (eds.): Digital Specimen 2014 – Abstracts of Presentations. Museum für Naturkunde Berlin. – [http://www.naturkundemuseum-berlin.de/forschung/tagungen/digitalspecimen-berlin-2014/home/abstracts-of-presentations/ Abstract] and [http://www.snsb.info/PDFs/Werner-et-al_2014_Stacking_MovableTable.pdf Poster].