Hochauflösende Kameraeinrichtung mit softwaregesteuert bewegbarem Tisch optimiert für dreidimensionale Makroobjekte (Kleinpilze) und hohe Schärfentiefe

From GBIF.DE IT Wiki
Jump to: navigation, search

High-resolution camera system with software-controlled movable table optimized for three-dimensional macro objects (small fungi) and high depth of field

640px-Macro3.jpg High Resolution Camera System Example 2013-09-26.jpg High Resolution Camera System Example2 2013-09-27.jpg

Camera hardware

  • DSLR: Nikon D2X (12.5 Megapixel)
  • Macro lens: Micro Nikkor 60 mm 1:2.8 D


Until December 2014:

  • 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter)
  • 2 x LED-5001 Video Light (5500 K, 700 lx, diffusor filter)

Since January 2015:

  • 2 x F&V 5600K-3200K Bi-color Flood K4000s LED Studio Light Panel (diffusor filter)
  • 1 x CN-126 LED Video Lighting (5400 K, 650 lx, diffusor filter)

Digitization workstation - hardware and software

Computer hardware:

  • Intel Core i7-870 CPU (8M Cache, 2.93 GHz)
  • 8 GB RAM
  • 2 x Western Digital WD5002ABYS-02B1B0 (500 GB, SATA2) RAID 0 HDD
  • NVIDIA Quadro 2000 graphics card (1 GB DDR5 frame buffer)
  • QUATO Intelli Proof 242 ex 24" proof monitor
  • Handheld barcode scanner (USB)

Electric sample table (XYZ-Sample-Scanner):

  • Software-controlled table (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreuth), automatically adjustable in three dimensions with micrometre precision by means of electrical stepping motors


  • Windows 7 Ultimate 64 Bit
  • Nikon Camera Control Pro 1.3.0
  • Elub 0781 2.1 control software (custom product by P. Reiß, Elektronikwerkstatt Universität Bayreuth)
  • PhotoAcute 3
  • Adobe Photoshop 12.0.4

Digitizing server infrastructure

Storage server for images, image web publication server, web server, database server - at the SNSB IT Center

Digitizing workflow

  • The object to be digitized is placed on the electric table.
  • Up to 30 single photographs are automatically taken by the Elub 0781 software thereby slightly changing the focus settings after each shot according to predefined parameters. This procedure takes about 10 minutes.
  • The single shots (RAW files) are further processed to one new image applying superresolution and focus stacking technologies provided by PhotoAcute, which takes about 20 minutes.
  • The resulting artificial image contains only that parts of the underlying single shots, which are in focus (focus stacking), and its resolution is enhanced approximately fourfold (superresolution).
  • In Photoshop, this final raw image file is color-adjusted, cropped, and exported as TIFF, which takes about 5 minutes.
  • Single shot raw files, combined raw file, and TIFFs are stored at the Leibniz Rechenzentrum München.

Contact person(s)

Sebastian Werner

Dr. Tobias Lehmann

Dr. Konstanze Bensch


  • Werner, S., Bensch, K., Beck, A., Neubacher, D., Reichert, W., Reiß, P. & Triebel, D. 2014. Multi-layered digitization of research-relevant objects using focus stacking technologies and a software-controlled movable table. – In: Mallison, H., Vogel, J. & Belvedere, M. (eds.): Digital Specimen 2014 – Abstracts of Presentations. Museum für Naturkunde Berlin. – Abstract and Poster.